Spatial
Proteomics 2
Capabilities
CyTOF® XT System allows researchers to dive deep into single-cell proteomics by detecting over 50 markers
Technology
CyTOF® XT System allows researchers to dive deep into single-cell proteomics by detecting over 50 markers
Workflow
CyTOF® XT System allows researchers to dive deep into single-cell proteomics by detecting over 50 markers
Overview
Understanding the intricate behavior of individual cells is critical in today’s biomedical research, especially for immune system analysis, disease progression, and therapeutic response.
Imaging Mass Cytometry™ (IMC™)
The dynamics of tissue microenvironments play a crucial role in understanding disease progression, therapeutic response, and biomarker discovery. Imaging Mass Cytometry™ (IMC™) technology, powered by the Hyperion™ XTi Imaging System, enables researchers to explore the complexity of these dynamics with unparalleled precision. By detecting over 40 protein and RNA markers simultaneously in tissue samples, IMC uncovers spatial relationships that conventional imaging methods overlook.
Designed for high throughput and reproducibility, IMC offers the highest dynamic range in the industry, making it ideal for translational and clinical research. Whether focusing on oncology, immunology, or other complex diseases, these spatial proteomics solutions deliver the detailed insights needed to guide any phase of research.
Discover the throughput and precision that is uniquely designed for translational researchers.
Flow is a disadvantaged biomarker screening tool where CyTOF is the widest screening tool capturing the most relevant immune metrics.
Traditional flow cytometry is constrained
Our Work
CyTOF XT detects over 50 markers at once, far exceeding the limits of traditional flow cytometry. This enables a comprehensive analysis of immune populations and functional responses in a single experiment.
No Autofluorescence Interference
IMC utilizes metal-tagged antibodies instead of fluorophores, eliminating issues with autofluorescence and delivering cleaner, more reliable data, even from challenging tissue samples.
High Dynamic Range
Our Work
Unlike traditional fluorescence-based imaging techniques, which often struggle with signal saturation and autofluorescence, IMC captures both high and low levels of protein expression in the same sample with exceptional clarity.
Assay Development Time
Our Work
IMC allows for the simultaneous imaging of over 40 markers in a single tissue section, facilitating the detailed mapping of tissue architecture, immune cell infiltration, and biomarker expression. This multiplexing capability provides richer, more comprehensive data with fewer processing cycles than traditional methods.
Simultaneous Detection of 40+ Markers
Our Work
Because IMC technology uses an antibody reagent cocktail, it is simple to mix and match different antibodies without extensive assay validation.
Flexibility of tissue acquisition
Our Work
Flexibility of tissue acquisition parameters (Hyperion imaging modes) and Automated, High-Throughput Imaging: With the Hyperion XTi™ Slide Loader, researchers can image up to 40 slides in 24 hours, making it easier to process large-scale studies and accelerate results without compromising precision – run an entire study in an automated, walkaway run.
No Autofluorescence Interference
Our Work
IMC utilizes metal-tagged antibodies instead of fluorophores, eliminating issues with autofluorescence and delivering cleaner, more reliable data, even from challenging tissue samples.
Against the Status Quo
Traditional
Fluorescence-Based Imaging
Traditional imaging methods, such as cyclic immunofluorescence, are limited by their low dynamic range, autofluorescence interference, and slow, multi-cycle processes. For example, brightfield imaging (DAB) gives at best one order of magnitude (OoM) of dynamic range, while fluorescence gives 2–3 OoM and IMC gives 5 OoM. These limitations often result in incomplete data, as they cannot capture both high- and low-expressing biomarkers in a single image, leading to missed insights.
Spectral Overlap
< 20 Markers
Staining Redundancy
Our Work
Standard Bio
Fluorescence-Based Imaging
IMC overcomes these challenges by capturing the full dynamic range of spatial biomarkers, detecting both high- and low-expressing proteins simultaneously. This approach eliminates the need for multiple imaging cycles and delivers high-resolution, quantitative data without the drawbacks of autofluorescence. IMC provides up to 100 times higher throughput than cyclic fluorescence, making it the ideal solution for large-scale research studies.
Spectral Overlap
< 20 Markers
Staining Redundancy
Technology
Imaging Mass Cytometry™ (IMC™) is the most trusted technology that enables researchers to accurately assess complex phenotypes and immune spatial interactions in the tissue microenvironment.
Unique scale and high signal resolution
What sets us apart
Unique scale and high signal resolution
Simultaneously detect over 50 markers without spectral overlap
Higher-content, more reliable dataset in a single analysis
Faster, more accurate insights into complex cellular behaviors.
Hear how the team at Navignostics is translating spatial insights into the clinic by leveraging spatial proteomics into reporting within 72 hours of sample receipt, revolutionizing treatment decisions for each cancer patient.
Hyperion™ Imaging Systems use a one-step staining and detection approach that enables samples to be simultaneously stained, acquired and analyzed.
Modularized Panels
Swap markers without panel revalidation.
Simultaneous staining
Stain 40-plus markers for all slides at once.
One-step detection
Simultaneous imaging of 40-plus markers, including protein and RNA.
Precise signals
Image any tissue without autofluorescence.
Real-time analysis
Visualize 40-plus markers in 30 minutes.
Our Work
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Biomarker Discovery
Identify therapeutic targets that may be relevant to developing treatment strat…
Cell segmentation
See how the use of cell segmentation facilitates an end-to-end workflow for …
Dual Mode
Use your Hyperion XTi Imaging System to perform high-multiplex analysis of b…
Immuno-Oncology
Discover the impact of new immune therapies including checkpoint inhibit…
Infectious Disease
Learn about the use of IMC technology in COVID-19 research and other infect…
Phenotyping
Understand the presence and location of stromal, tumor and immune cells an…
Our Work